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Compact Line

Abberior Instruments is known for extraordinary customization ability of its flexible STED microscope platform. We have developed the ultimate compact STED and confocal microscope, called STEDYCON.


STEDYCON is a completely new class of nanoscope. It converts your conventional epifluorescence microscope into a versatile four-color confocal (405nm, 488nm, 561nm, 640nm) and STED (775nm) system! At the same time, it is incredibly compact and can be used by anyone! TEDYCON is a completely new class of nanoscope. It converts your conventional epifluorescence microscope into a versatile four-color confocal (405nm, 488nm, 561nm, 640nm) and STED (775nm) system! At the same time, it is incredibly compact and can be used by anyone!


Convert your existing microscope - Instantly upgrade any epifluorescence microscope body to multicolor confocal and STED capabilities. The STEDYCON has a variable pinhole and up to four detectors with superior DynamicPLUS Imaging.

Super-stable - Our new STEADYFOCUS module ensures that your images are perfectly in focus over days.

Get top-notch STED performance - With STEDYCON your microscope will image at 30nm resolution.

Save Space - The incredibly compact STEDYCON comes at the size of a camera.

Plug & Play - The STEDYCON is assembled within minutes. It simply connects to the C-mount camera port of your microscope with no need for optical alignment.

Service and maintenance free - The laser beams of the STEDYCON are aligned by design as provided by its patent-protected 'easySTED' optical arrangement.





STEADYFOCUS module


Our new push-button STEADYFOCUS module is a continuous laser-based hardware focus lock for the STEDYCON.


It guarantees confocal and STED imaging over days without focus drift.


On top, thanks to its proprietary design, it uniquely requires no additional optics in the imaging beam path, and therefore is 100% free of fluorescence losses or imaging distortions.


The STEADYFOCUS module is compatible with a multitude of immersion (water, oil, silicone, glycerol, ...) and embedding media (water-based, Mowiol, ...) and with upright and inverted microscope bodies of all major brands.




Examples of multicolor live-cell time-lapse confocal imaging using the STEADYFOCUS over many hours (up to 13 hrs).

Collaboration SVI and Abberior Instruments




Our fruitful collaboration with SVI has brought about cutting-edge superresolution at maximum convenience and benefit for our STEDYCON users.


As exemplified in the panels below, the combination of top-notch STED imaging with a sophisticated deconvolution method reveals the faintest details in the sample. Moreover, all this unfolds at the push of a button!

DynamicPLUS


Superior sensitivity and full dynamic range within the same image

Our new DynamicPLUS feature offers unrivaled performance with both STED and classical confocal applications. With DynamicPLUS, you can be sure to capture everything from the faintest details to the brightest spots.

The underlying avalanche photo detectors (APDs) have a superior quantum efficiency of up to 69% (@ 650nm) in the red fluorescence regime. This means that even when signal-levels are low, our APDs still collect plenty of photons for a meaningful image. Typical applications are super-resolution STED imaging and experiments with low labeling densities designed to stay close to physiological conditions.

At the same time, the dead-time compensation of DynamicPLUS allows crisp images of high-signal samples, such as bright confocal images. Of course, raw data for quantitative analysis and deconvolution is always available.




For Anyone


Our STEDYCON nanoscope works with all kinds of microscope bodies from all major vendors:



STEDYCON @ Olympus IX83
STEDYCON @ Olympus IX83
STEDYCON @ Olympus IX83
STEDYCON @ Olympus IX83
STEDYCON @ Olympus IX83
STEDYCON @ Olympus IX83
STEDYCON @ Olympus IX83
STEDYCON @ Olympus IX83
STEDYCON @ Olympus IX83
STEDYCON @ Olympus IX83



Browser-based cross-platform software


The STEDYCON can be controlled via Abberior Instruments STEDYCON smart control, a web-browser-based graphical user interface. STEDYCON smart control was designed from scratch to provide the highest possible usability completing the overall STEDYCON concept of easy installation, intuitive usage, and maintenance-free operation. Beginners can image with super-resolution within minutes. Smart control runs on every device with a modern web browser like Windows-based PCs, Linux-based PCs, Apple PCs or even tablets.



Top-Perfomance


Gattaquant STED-AFM ruler (70 nm distance between emitters) were imaged using a STEDYCON attached to a Nikon Eclipse Ni and a Zeiss Axio Imager Z2. Shown is RAW DATA

Videos

Three color confocal z-Stack of mammalian cells. Z-plane distance: 250 nm. Labelled structures: Actin cytoskeleton (blue, Phalloidin, Oregon Green 488), Nuclear pore complex (green, NUP153, Abberior STAR 580), Mitochondria (green, Tom20, Abberior STAR 635P). Measured @ STEDYCON/ Zeiss body




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